mouse ig standard  (SouthernBiotech)

Journal: The World Allergy Organization Journal

Article Title: High protein diet increases the risk of allergic sensitization but not asthma in mice through modulation of the cytokine milieu toward Th2 bias ☆

doi: 10.1016/j.waojou.2025.101031

Figure Lengend Snippet: Effect of feeding mice for 7 weeks either normal diet or high protein diet on serum levels of (A) OVA-specific IgE (B) OVA-specific IgG1 and (C) OVA-specific IgG2a from OVA-sensitized mice (n = 11 in each group) after OVA intranasal challenge. The high protein diet group showed higher levels of both OVA-specific IgE and IgG1compared to the normal diet group. ns: not significant, CI: confidence interval. IQR: interquartile range.

Article Snippet: After washing the wells, blocking buffer (0.5% gelatin in wash buffer or 3% Rat serum in wash buffer) was added and the plates incubated for 2 h. Appropriate reference standard OVA-IgE (Chondrex #3006) or OVA-IgG1 (Chondrex # 7093) or OVA-IgG2a (Chondrex # 7095) and diluted serum samples were then added to the wells and incubated for 3 h. This was followed by incubation with OVA-Biotin for 1 h and with streptavidin-HRP for 30 min. Tetramethylbenzidine substrate was added and the enzyme-substrate reaction stopped after 15 min, and the optical density measured at 450 nm.

Techniques:

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from S . Typhimurium induced by the regulated delayed attenuated Salmonella .Serum and vaginal secretions of mice were gathered at week 8 post-initial vaccination. Quantitative ELISA was applied to analyze the levels of specific IgG (a), IgA (b), IgG1 (c), and IgG2a (d) against S . Typhimurium OMPs. The results displayed the precise levels of antibodies, as measured by a standard curve, in mice orally inoculated with the regulated delayed attenuated Salmonella at the scheduled weeks. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a, b, and c indicate p < 0.05 for comparisons with the BSG, SW112 ( waaG ), and SW116 ( waaJ ) groups, respectively.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from S . Choleraesuis and S . Enteritidis induced by the regulated delayed attenuated Salmonella .Serum and vaginal secretions of mice were gathered at week 8 post-initial vaccination. Quantitative ELISA was applied to analyze the levels of specific IgG (a, c) and IgA (b, d) against OMPs from S . Enteritidis and S . Choleraesuis, as well as the subtypes IgG1 (e, g) and IgG2a (f, h). The results displayed the precise levels of antibodies, as measured by a standard curve, in mice orally inoculated with the regulated delayed attenuated Salmonella at the scheduled weeks. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a, b, and c indicate p < 0.05 for comparisons with the BSG, SW114 ( waaI ), and SW120 ( wbaP ) groups, respectively.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from other enteric bacteria induced by the regulated delayed attenuated Salmonella .The sera were gathered from mice in SW114 ( waaI ), SW116 ( waaJ ), and SW118 ( waaL ) groups on days 42 after the primary immunization. Quantitative ELISA was applied to analyze the levels of specific IgG against OMPs from E. coli O8 (A), E. coli O138 (B), and E. coli O139 strain (C). The results displayed the precise levels of antibodies, as measured by a standard curve. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a indicate p < 0.05 for comparisons with the BSG groups.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay